University of Leeds
The Astbury Centre at University of Leeds brings together researchers from across the University to allow interdisciplinary approaches to be harnessed to understand the molecular basis of life. The Astbury Biostructure Laboratory (ABSL), created by a £17M University of Leeds investment in 2015, assembles specialist top-end structural biology capability (950 MHz NMR and two 300keV Titan Krios cryo-electron microscopes, plus state-of-the-art structural MS) for pushing forward the frontiers of structural molecular biology research. ABSL is a member of Instruct-ERIC, a pan-European structural biology research infrastructures consortium that provides subsidised access to high-end capabilities.
Prof. Alex Breeze
Ultra-High Field (>800 MHz)
Bruker Avance III HD 950 MHz narrow bore NMR spectrometer actively shielded and with helium recycling technology, with 4 channels and dual receivers, equipped with automatic sample changer for up to 24 NMR tubes.
Solution state probes:
- 5 mm triple resonance (15N, 13C, 1H, 2H TXO) He-cooled cryoprobe, z-gradients, cold amplifiers on 15N, 13C, 1H and 2H, optimised for 15N and 13C direct detection experiments with best available C/N sensitivity
- 3 mm 1H-optimised triple resonance (1H, 13C, 15N, 2H TCI) He-cooled cryoprobe, z-gradients, cold amplifiers on 13C, 1H and 2H, optimised for salt tolerance at lower sample volumes